Wheatgrass Nutritional Analyses (Leaf)

12 Sept 2002 Certificate of Analysis Samples Analysed Barley Grass Leaf Powder Wheat Leaf Powder Date Analysed 4 August 2002 Crop & Food Reference JTB-BG1 & JTB-WL1 Results Chlorophyll determined spectrophotometrically Carotenoids determined spectrophotometrically as ß-carotene equivalents Sample ID Analsis mg/g Barley Grass Carotenoids 0.71 Chlorophyll 9.82 Wheat Leaf Carotenoids 1.04 Chlorophyll 9.07

Comments: Methods for determination of chlorophyll and carotenoid content (ß-carotene equivalents) adapted from: Knee, M. 1972: Anthocyanin, carotenoid, and chlorophyll changes in the peel of Cox's Orange Pippin apples during ripening on and off the tree. Journal of Experimental Botany 23: 184-196.

Analyst Carolyn Lister Qualifying StatementThe results given in this report apply only to the samples provided to Crop and Food Research, which may or may not be representative of the entire samples of plant material.

The analysis was done by Crop and Food Research NZ

Wheat Grass Nutritional Analyses

Results apply to samples as received

	Units	Amount
Energy	kJ/100g	1064.5
Nitrogen	%	4.54
Protein	g/100g	28.38
Fat – total	g/100g	6.03
Fat – saturated
Lauric acid (12:0)	g/100g	0.02
Palmitic acid (16:0)	g/100g	0.47
Stearic acid (18:0)	g/100g	0.02
Behenic acid (22:0)	g/100g	0.03
Fat – unsaturated
Oleic acid (18:1)	g/100g	0.04
Linoleic acid (18:2)	g/100g	0.45
Linolenic acid (18:3)	g/100g	3.17
Fat – unidentified	g/100g	1.83
Carbohydrate – total	g/100g	4.92 [1]
Carbohydrate – starch	g/100g	0.32
Carbohydrate – sugars
Fructose	g/100g	0.6
Glucose	g/100g	1.2
Lactose	g/100g	<0.1
Maltose	g/100g	<0.1
Sucrose	g/100g	2.8
Fibre [2]	g/100g	34.41
Aluminium	mg/100g	13
Arsenic	mg/100g	<25
Boron	mg/100g	680
Cadmium	mg/100g	1
Calcium	mg/100g	430
Cesium	mg/100g	4.1
Chromium	mg/100g	210
Cobalt	mg/100g	6.1
Copper	mg/100g	930
Iron	mg/100g	17
Lead	mg/100g	16
Lithium	mg/100g	11
Magnesium	mg/100g	170
Manganese	mg/100g	2300
Mercury	mg/100g	<2
Molybdenum	mg/100g	53
Nickel	mg/100g	60
Phosphorus	mg/100g	410
Potassium	mg/100g	3800
Rubidium	mg/100g	39
Selenium	mg/100g	<25
Sodium	mg/100g	320
Tin	mg/100g	3.8
Vanadium	mg/100g	33
Zinc	mg/100g	2900
Vitamin C	mg/100g	548
Vitamin E	IU/100g	36.1
Thiamine	mg/100g	0.80
Vitamin B2 &#8211; total	mg/100g	2.33
Vitamin B3 &#8211; total	mg/100g	6.49
Vitamin B6 &#8211; total	mg/100g	1.30
Folic acid	mg/100g	1130
Beta-carotene	mg/100g	<10

Amino Acid Profile

Free Amino Acids	mg/g	% weight [3]
D,L-O-Phosphoserine	ND [4]	0.00
Taurine	ND	0.00
O-Phosphoethanolamine	ND	0.00
L-Aspartic acid	0.50	2.91
L-Hydroxyproline	ND	0.00
L-Threonine	0.58	3.35
L-Serine	1.07	6.21
L-Asparagine	4.32	24.97
L-Glutamic acid	0.61	3.53
L-Glutamine	1.43	8.27
D,L-a-Aminoadipic acid	ND	0.00
L-Proline	0.33	1.93
Glycine	0.14	0.80
L-Alanine	1.69	9.80
Citrulline	ND	0.00
L-a-Amino-n-butyric acid	ND	0.00
L-Valine	0.56	3.23
L-Cystine	ND	0.00
L-Methionine	0.15	0.85
L-Cystathione	ND	0.00
L-Isoleucine	0.27	1.59
L-Leucine	0.49	2.86
L-Norleucine	ND	0.00
L-Tyrosine	0.18	1.05
L-Phenylalanine	0.39	2.28
b-Alanine	ND	0.00
D,L-b-Amino-i-butyric acid	0.55	3.16
D,L-Homocystine	ND	0.00
g-Amino butyric acid	3.10	17.90
L-Tryptophan	ND	0.00
Ethanolamine	ND	0.00
D,L & allo-Hydroxylysine	ND	0.00
Ammonia	0.09	0.51
L-Ornithine	ND	0.00
L-Lysine	0.26	1.50
L-Histidine	0.29	1.70
L-3-Methylhistidine	ND	0.00
L-1-Methylhistidine	ND	0.00
L-Arginine	0.28	1.61
TOTAL	17.29	100.00

Amino Acid Pro

After Hydrolysis	mg/g	% weight [5]
Alanine	16.0	7.1
Arginine	14.3	6.4
Aspartic acid [6]	29.1	13.0
Glutamic acid6	29.8	13.3
Glycine	11.9	5.3
Histidine	5.3	2.4
Isoleucine	8.9	4.0
Leucine	19.3	8.6
Lysine	15.2	6.8
Phenylalanine	12.3	5.5
Proline	12.2	5.4
Serine	11.7	5.2
Threonine	11.8	5.3
Tyrosine	9.2	4.1
Valine	16.8	7.5
TOTAL	224.1	100.0

[1] Minimum value

[2] Total dietary fibre

[3] Relative amount of each amino acid as a percentage of the total reported

[4] ND: not detected

The results for aspartic acid and glutamic acid may include contribution from asparagines and glutamine that have been converted to aspartic acid and glutamic acid during acid hydrolysis

References:

http://www.gogreen.net.nz/wheatgrass-table.htm